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Geoengineering Watch Global Alert News, November 30, 2024, #486
Dane Wigington GeoengineeringWatch.org "The U.S. Is Building an Early Warning System to Detect Geoengineering" (from The -
Geoengineering Watch Global Alert News, November 23, 2024, #485
Dane Wigington GeoengineeringWatch.org "Bomb cyclone pummels Northern California with heavy snow and record rain, knocks out -
“Bomb Cyclone”, 90 Second Alert
Dane Wigington GeoengineeringWatch.org "California’s rainy season begins with a bomb cyclone bang" (CNN). "Major storm drops -
Geoengineering Watch Global Alert News, November 16, 2024, #484
Dane Wigington GeoengineeringWatch.org Chemical winter, "Microplastics: Unexpected Ice Makers in the Sky" (from ZME Science). "Startling New -
Geoengineering Watch Global Alert News, November 9, 2024, #483
Dane Wigington GeoengineeringWatch.org "Winter Storm Anya Spread Heavy Snow To Colorado And New Mexico" (MSN). "Blizzard: -
Geoengineering Watch Global Alert News, November 2, 2024, #482
Dane Wigington GeoengineeringWatch.org "Will flooding, storms play roles in swing states on Election Day 2024?" (AccuWeather), -
Geoengineering Watch Global Alert News, October 26, 2024, #481
Dane Wigington GeoengineeringWatch.org "ScienceAdviser: Geoengineering in the sky with diamonds" (science.org). Seriously? From Yahoo News: "high -
Geoengineering Watch Global Alert News, October 19, 2024, #480
Dane Wigington GeoengineeringWatch.org From unnatural firestorms to chemical cooldowns, "Global water cycle off balance for first -
Geoengineering Watch Global Alert News, October 12, 2024, #479
Dane Wigington GeoengineeringWatch.org "Weather Wars: If states decided unilaterally to use more radical geoengineering technologies it -
Geoengineering Watch Global Alert News, October 5, 2024, #478
Dane Wigington GeoengineeringWatch.org "October hurricane forecast: Brace for the return of big hurricanes" (USA Today). "Milton
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Engineered Droughts
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Wildfires As A Weapon: US Military Exposed
Dane Wigington GeoengineeringWatch.org Is the military industrial complex insane enough to incinerate Earth's last remaining forests -
Drilling Under Lake Mead To Drain The Last Drop
Dane Wigington GeoengineeringWatch.org Many are now aware of and justifiably concerned about Lake Mead soon becoming -
Domino Effect: Weather Warfare, Wasted Forests and Worldwide Collapse Of Ecosystems
Dane Wigington GeoengineeringWatch.org The global controllers are utilizing every tool they have at their disposal to -
California Drought, How To Party On The Titanic
Dane Wigington GeoengineeringWatch.org Climate engineering operations are cutting off the flow of moisture to the US -
Geoengineering: Fire And Ice
Dane Wigington GeoengineeringWatch.org Geoengineering is wreaking havoc all over the world, from fires to ice. After -
Geoengineering Creating Freeze Fry Extremes
Dane Wigington GeoengineeringWatch.org Global climate engineering operations are creating ever more extreme weather and temperature whiplash -
Governor Newsom, Geoengineering And Unprecedented Wildfires
Dane Wigington GeoengineeringWatch.org Newly elected California governor Gavin Newsom has had to declare state of emergency due to -
Geoengineering Is Fueling Firestorm Catastrophes
Dane Wigington GeoengineeringWatch.org Yet again forests in the Western US are incinerating as the US east -
Global Weather Modification Assault Causing Climate Chaos And Environmental Catastrophe
Dane Wigington geoengineeringwatch.org Global climate engineering encompasses many different aspects and processes. The consequences of the -
NASA, And The Criminal Denial Of Climate Engineering
Dane Wigington GeoengineeringWatch.org NASA is nothing more than a tool for the military industrial complex to
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Radio
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Geoengineering Watch Global Alert News, November 30, 2024, #486
Dane Wigington GeoengineeringWatch.org "The U.S. Is Building an Early Warning System to Detect Geoengineering" (from The New York Times). How absurd is that? To monitor themselves? "Major lake-effect snow -
Geoengineering Watch Global Alert News, November 23, 2024, #485
Dane Wigington GeoengineeringWatch.org "Bomb cyclone pummels Northern California with heavy snow and record rain, knocks out power for hundreds of thousands in Seattle" (PBS). "Atmospheric river dumps -
Geoengineering Watch Global Alert News, November 16, 2024, #484
Dane Wigington GeoengineeringWatch.org Chemical winter, "Microplastics: Unexpected Ice Makers in the Sky" (from ZME Science). "Startling New Research Reveals That Microplastics Could Be Changing Earth’s Climate" (from SciTechDaily). -
Geoengineering Watch Global Alert News, November 9, 2024, #483
Dane Wigington GeoengineeringWatch.org "Winter Storm Anya Spread Heavy Snow To Colorado And New Mexico" (MSN). "Blizzard: Feet Of Snow Cover New Mexico" (The Weather Channel). "Saudi Arabian -
Geoengineering Watch Global Alert News, November 2, 2024, #482
Dane Wigington GeoengineeringWatch.org "Will flooding, storms play roles in swing states on Election Day 2024?" (AccuWeather), "U.S elections: Weather could play spoilsport in these three swing states -
Geoengineering Watch Global Alert News, October 26, 2024, #481
Dane Wigington GeoengineeringWatch.org "ScienceAdviser: Geoengineering in the sky with diamonds" (science.org). Seriously? From Yahoo News: "high levels of ultrafine particles in the atmosphere over long periods of -
Geoengineering Watch Global Alert News, October 19, 2024, #480
Dane Wigington GeoengineeringWatch.org From unnatural firestorms to chemical cooldowns, "Global water cycle off balance for first time in human history, threatening half the planet’s food production" (CNN). "Scientists -
Geoengineering Watch Global Alert News, October 12, 2024, #479
Dane Wigington GeoengineeringWatch.org "Weather Wars: If states decided unilaterally to use more radical geoengineering technologies it could trigger dramatic climatic disruptions" (from the World Economic Forum). For -
Geoengineering Watch Global Alert News, October 5, 2024, #478
Dane Wigington GeoengineeringWatch.org "October hurricane forecast: Brace for the return of big hurricanes" (USA Today). "Milton expected to become a major hurricane before slamming into Florida" (Fox -
Geoengineering Watch Global Alert News, September 28, 2024, #477
Dane Wigington GeoengineeringWatch.org "People should not be there: 'Unsurvivable' 20-foot storm surge predicted as ferocious Hurricane Helene heads to Florida" (from Live Science). Was Hurricane Helene everything official
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Creating Storms
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“Bomb Cyclone”, 90 Second Alert
Dane Wigington GeoengineeringWatch.org "California’s rainy season begins with a bomb cyclone bang" (CNN). "Major storm drops record rain, downs trees in Northern California after devastation further north" -
Hurricane Helene And Frequency Transmissions, 90 Second Alert
Dane Wigington GeoengineeringWatch.org Was Hurricane Helene’s path and behavior just an act of nature? What do you think? Are networks of ground based frequency transmitters being utilized -
Was Hurricane Debby’s Path Manipulated?
Dane Wigington GeoengineeringWatch.org Was Hurricane Debby's path manipulated? Are we actually expected to believe the official denial of climate engineering / hurricane modification operations when the historical -
Manufacturing Winter Blizzard
Dane Wigington GeoengineeringWatch.org Chemically nucleated “snow” events are wreaking havoc on forests, ecosystems and human infrastructure. Chemical ice nucleation cloud seeding processes are a primary component of the ongoing -
The “Unprecedented” Scheduled Path Of Hurricane Idalia
Dane Wigington GeoengineeringWatch.org Did Hurricane Idalia follow a trajectory that was laid out by nature? Or is there much more to the equation of Idalia's path? The
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Aircraft Spraying Videos
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Engineering Winter: The Untold Story Of Hurricane Nicole
Dane Wigington GeoengineeringWatch.org Was hurricane Nicole a random act of nature? Or is there much more to the story? What agendas and objectives might Hurricane -
Graphene Skies?
Dane Wigington GeoengineeringWatch.org What aren’t we being told? Is the highly toxic and controversial element graphene being seeded into our skies as part of the ongoing covert -
Geoengineering Watch: Our First Ever High Altitude Atmospheric Testing
Dane Wigington GeoengineeringWatch.org After substantial difficulty and expense, Geoengineering Watch has utilized two types of aircraft to complete multiple atmospheric particulate sampling flights up to -
Climate Engineering, New Film Footage Confirmation
Dane Wigington GeoengineeringWatch.org The climate science community is increasingly calling for massive global climate engineering / solar radiation management operations to be immediately deployed to -
Geoengineering Reality: Film Footage And Facts Prove Contrail Myth
Dane Wigington GeoengineeringWatch.org Sharing shocking and inarguable film footage of jet aerosol spraying is the most powerful tool of all for waking people up to -
Geoengineering, Weather Warfare, And The Contrail Deception
Dane Wigington GeoengineeringWatch.org Official sources, who’s job it is to deny the blatantly obvious climate engineering atrocities, have consistently claimed that the vapor trails seen behind WWll -
NASA, And The Criminal Denial Of Climate Engineering
Dane Wigington GeoengineeringWatch.org NASA is nothing more than a tool for the military industrial complex to carry out their nefarious agendas and to cover up their
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Activist Articles & Videos
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Geoengineering Watch Global Alert News, June 19, 2021, #306
Dane Wigington GeoengineeringWatch.org From the weather, to the behavior of societies, to unfolding controller agendas, all is rapidly becoming more extreme and all is connected. The gravity -
Geoengineering Watch Global Alert News, December 14, 2019, #227
Dane Wigington GeoengineeringWatch.org Ongoing investigations and document releases continue to expose the endless flow of lies and deception emanating from the US government. Endless political theater is -
All The Way – A Charles Bukowski Poem
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Geoengineering Watch Global Alert News, September 22, 2018, #163
Dane Wigington GeoengineeringWatch.org From all over the world the headlines continue to pour in, change is occurring at blinding speed. As our forests and wildlife disappear, instead -
Sowing Seeds
What are we to do as the walls close in on us? Is it not our duty of care to protect our children's future and this -
Mirror, Mirror In The Sky
By Irene Parousis “We’re going to build a mirror factory first and put out nothing but mirrors for the next year and take a long look in them." – Fahrenheit
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Health
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Graphene Rain, Scientist Sounds Alarm
Dane Wigington GeoengineeringWatch.org Graphene rain, are climate engineering operations the source? What aren't we being told? A highly credentialed scientist provides extensive analysis, this is a must watch report. -
Nanoparticle Contamination Cover-Up: Answers From A Scientist
Dane Wigington GeoengineeringWatch.org A must view report from a top scientist covering the climate engineering nanoparticle pollution of our air, water, soils and food supply. All are -
Climate Engineering Contamination, Staying Healthy In A World That Isn’t
Dane Wigington geoengineeringwatch.org How does one retain some semblance of health while existing in a world that has become alarmingly toxic and incompatible to life as a whole? Though -
US EPA Scientist Fired For Trying To Tell The Truth About Climate Engineering And Fluoridated Water
Dane Wigington GeoengineeringWatch.org The public has been trained and conditioned to believe that federal agencies like the EPA exist to watch over them and warn them of -
World-Renowned Doctor Addresses Climate Engineering
Dane Wigington GeoengineeringWatch.org How do we retain our health in an increasingly contaminated environment? Though there are countless forms of contamination from human activities, the climate engineering -
The Great Nutrient Collapse
Source: Politico Irakli Loladze is a mathematician by training, but he was in a biology lab when he encountered the puzzle that would change his life. -
Weather Warfare Assault, A Physician Speaks Out
The ongoing battle to expose and halt the climate engineering insanity (by reaching a critical mass of awareness on the issue) is now rapidly gaining traction. -
Covering Up Climate Engineering Contamination, “EPA Caught Buying It’s Independent Science Advisors”
Dane Wigington geoengineeringwatch.org A lawsuit has been filed to expose the EPA for awarding $190 million in taxpayer grants to "independent" scientists in order to get the -
New Science Study Comes Closer To Disclosing The Catastrophic Health And Environmental Consequences Of Climate Engineering
Dane Wigington geoengineeringwatch.org The walls of the power structure are beginning to buckle, those that have served the power brokers are beginning to respond to their own instinct -
Health Magazine Covers Climate Engineering: “Heavy Metal Contamination And Mass Extinction – What’s The Connection?”
As the global climate system continues to unravel at an ever more rapid pace, the effort to sound the alarm is also gaining ground. Health Freedom News
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Tree Die-off
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Geoengineering Watch Global Alert News, April 15, 2023, #401
Dane Wigington GeoengineeringWatch.org This week there were regions of the US that went from daily record high temperatures pushing 90 degrees or more to snow in the -
Nanoparticle Contamination Cover-Up: Answers From A Scientist
Dane Wigington GeoengineeringWatch.org A must view report from a top scientist covering the climate engineering nanoparticle pollution of our air, water, soils and food supply. All are -
Wildfires As A Weapon: US Military Exposed
Dane Wigington GeoengineeringWatch.org Is the military industrial complex insane enough to incinerate Earth's last remaining forests in order to achieve the objectives of the global controllers? The -
Domino Effect: Weather Warfare, Wasted Forests and Worldwide Collapse Of Ecosystems
Dane Wigington GeoengineeringWatch.org The global controllers are utilizing every tool they have at their disposal to distract populations from the wider horizon of unfolding and accelerating global -
Aftermath Of Chemical Ice Nucleation
Dane Wigington GeoengineeringWatch.org Chemically nucleated “snow” events are wreaking havoc on forests, ecosystems and human infrastructure. Chemical ice nucleation cloud seeding processes are a primary component of -
Chemically Nucleated Winter Weather
Dane Wigington GeoengineeringWatch.org The extremely anomalous surface cool-down in Texas and other parts of the world are not just random acts of Nature. Global climate engineering operations are not just a
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Radio Frequency Dangers
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Hurricane Helene And Frequency Transmissions, 90 Second Alert
Dane Wigington GeoengineeringWatch.org Was Hurricane Helene’s path and behavior just an act of nature? What do you think? Are networks of ground based frequency transmitters being utilized -
Was Hurricane Debby’s Path Manipulated?
Dane Wigington GeoengineeringWatch.org Was Hurricane Debby's path manipulated? Are we actually expected to believe the official denial of climate engineering / hurricane modification operations when the historical -
Climate Engineering, Microwave Transmissions, And The April Nor’easter
Dane Wigington GeoengineeringWatch.org Extremely powerful ground based microwave transmission facilities are being used in conjunction with the ongoing atmospheric aerosol spraying operations to completely manipulate Earth’s climate -
The Extreme Dangers Posed By “Smart Meters”
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Weather Terrorists Control The Climate
Dane Wigington GeoengineeringWatch.org Climate engineering is nothing short of weather and biological warfare. Not only are our skies being constantly saturated with highly toxic climate engineering elements,
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Perspectives
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All The Way – A Charles Bukowski Poem
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CYMATICS: Science Vs. Music – Nigel Stanford
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The Most Inspiring Speech: The Wisdom Of A Third Grade Dropout Will Change Your Life – Rick Rigsby
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“I Should Have Done Something” – From The Conspiracy Project
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We Are From The Future
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My Christmas Duty To Warn Column: Questioning Christian Participation In Homicide By Remembering The Christmas Truce Of 1914
Duty To Warn Remembering the Christmas Truce of 1914: (And Questioning Christian Participation in Homicide) How Soldiers with Newly Attuned Consciences Almost Stopped a War By -
Duty To Warn: Two Of The Culprits That Are Behind America’s Iatrogenic Over-Vaccination Monster
Duty To Warn The Culprits at the CDC and FDA That Helped Create America’s Iatrogenic Over-Vaccination Monster By Gary G. Kohls, MD December 4, 2017 Over -
Duty To Warn: Changing The “War No More” Sentiment Of Armistice Day To The War-Glorifying
Duty To Warn Changing the “War No More” Sentiment of Armistice Day to the War-Glorifying Propaganda of Veterans Day By Gary G. Kohls, MD 11-11-17 99 -
Geoengineering, The Ultimate Weapon In The Toxic War On Humanity And Mother Earth. How To Protect Yourself In This Escalating Battle
Source: Juan Matus The irreversible awaking of humanity on the fact that we are under attack by the psychopaths in power is exponentially ongoing. More and -
Geoengineering And The Most Dangerous False Dichotomy
By Penny Teal, PhD, contributing writer for geoengineeringwatch.org We all know despair. We all know joy. We all have been told to believe that these two states
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These Jets are Spraying Aerosols
Government Document: “Weather as a Force Multiplier”
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Previous Articles Archive
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Recent
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Geoengineering Watch Global Alert News, November 30, 2024, #486
Dane Wigington GeoengineeringWatch.org "The U.S. Is Building an Early Warning System to Detect Geoengineering" (from The New York Times). How absurd is that? To monitor themselves? "Major lake-effect snow -
Geoengineering Watch Global Alert News, November 23, 2024, #485
Dane Wigington GeoengineeringWatch.org "Bomb cyclone pummels Northern California with heavy snow and record rain, knocks out power for hundreds of thousands in Seattle" (PBS). "Atmospheric river dumps -
“Bomb Cyclone”, 90 Second Alert
Dane Wigington GeoengineeringWatch.org "California’s rainy season begins with a bomb cyclone bang" (CNN). "Major storm drops record rain, downs trees in Northern California after devastation further north" -
Geoengineering Watch Global Alert News, November 16, 2024, #484
Dane Wigington GeoengineeringWatch.org Chemical winter, "Microplastics: Unexpected Ice Makers in the Sky" (from ZME Science). "Startling New Research Reveals That Microplastics Could Be Changing Earth’s Climate" (from SciTechDaily). -
Geoengineering Watch Global Alert News, November 9, 2024, #483
Dane Wigington GeoengineeringWatch.org "Winter Storm Anya Spread Heavy Snow To Colorado And New Mexico" (MSN). "Blizzard: Feet Of Snow Cover New Mexico" (The Weather Channel). "Saudi Arabian -
Geoengineering Watch Global Alert News, November 2, 2024, #482
Dane Wigington GeoengineeringWatch.org "Will flooding, storms play roles in swing states on Election Day 2024?" (AccuWeather), "U.S elections: Weather could play spoilsport in these three swing states -
Geoengineering Watch Global Alert News, October 26, 2024, #481
Dane Wigington GeoengineeringWatch.org "ScienceAdviser: Geoengineering in the sky with diamonds" (science.org). Seriously? From Yahoo News: "high levels of ultrafine particles in the atmosphere over long periods of -
Geoengineering Watch Global Alert News, October 19, 2024, #480
Dane Wigington GeoengineeringWatch.org From unnatural firestorms to chemical cooldowns, "Global water cycle off balance for first time in human history, threatening half the planet’s food production" (CNN). "Scientists -
Geoengineering Watch Global Alert News, October 12, 2024, #479
Dane Wigington GeoengineeringWatch.org "Weather Wars: If states decided unilaterally to use more radical geoengineering technologies it could trigger dramatic climatic disruptions" (from the World Economic Forum). For -
Geoengineering Watch Global Alert News, October 5, 2024, #478
Dane Wigington GeoengineeringWatch.org "October hurricane forecast: Brace for the return of big hurricanes" (USA Today). "Milton expected to become a major hurricane before slamming into Florida" (Fox -
Hurricane Helene And Frequency Transmissions, 90 Second Alert
Dane Wigington GeoengineeringWatch.org Was Hurricane Helene’s path and behavior just an act of nature? What do you think? Are networks of ground based frequency transmitters being utilized -
Geoengineering Watch Global Alert News, September 28, 2024, #477
Dane Wigington GeoengineeringWatch.org "People should not be there: 'Unsurvivable' 20-foot storm surge predicted as ferocious Hurricane Helene heads to Florida" (from Live Science). Was Hurricane Helene everything official -
Geoengineering Watch Global Alert News, September 21, 2024, #476
Dane Wigington GeoengineeringWatch.org "Geoengineering is becoming a necessary option", so says a new report from Earth.com. The article continues by stating global climate engineering operations "might exacerbate droughts, -
Geoengineering Watch Global Alert News, September 14, 2024, #475
Dane Wigington GeoengineeringWatch.org "The Weather Gods Who Want Us to Believe They Can Make Rain on Demand" (from Mother Jones News). "Amazon rainforest drier than Sahara as drought -
Geoengineering Watch Global Alert News, September 7, 2024, #474
Dane Wigington GeoengineeringWatch.org "Frost Warning Issued for Three States as Temperatures Plunge. Cooler weather has hit the Northeast and the Midwest this week, with Montana seeing a
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Read More From “Recent” >>
1966 Government Weather Modification Document
Weather Warfare Government Documents
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Engineering Winter
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“Bomb Cyclone”, 90 Second Alert
Dane Wigington GeoengineeringWatch.org "California’s rainy season begins with a bomb cyclone bang" (CNN). "Major storm drops record rain, downs trees in Northern California after devastation further north" -
Aluminum Snow: Lab Test Confirmed
Dane Wigington GeoengineeringWatch.org Geoengineering Watch will start to release as of yet unseen footage from the filming of The Dimming, this is the first installment. Aluminum nanoparticle -
Engineering Winter: The Untold Story Of Hurricane Nicole
Dane Wigington GeoengineeringWatch.org Was hurricane Nicole a random act of nature? Or is there much more to the story? What agendas and objectives might Hurricane Nicole have -
Aftermath Of Chemical Ice Nucleation
Dane Wigington GeoengineeringWatch.org Chemically nucleated “snow” events are wreaking havoc on forests, ecosystems and human infrastructure. Chemical ice nucleation cloud seeding processes are a primary component of -
Chemically Nucleated Winter Weather
Dane Wigington GeoengineeringWatch.org The extremely anomalous surface cool-down in Texas and other parts of the world are not just random acts of Nature. Global climate engineering operations are not just a -
Engineering Climate Chaos
Dane Wigington GeoengineeringWatch.org Numerous record cold temperatures continue to occur in various locations of the US. How is is possible for such a consistently anomalous cooling of such a -
Geoengineering Creating Freeze Fry Extremes
Dane Wigington GeoengineeringWatch.org Global climate engineering operations are creating ever more extreme weather and temperature whiplash scenarios. The immense scale of climate forcing being carried out by -
Climate Engineering, Microwave Transmissions, And The April Nor’easter
Dane Wigington GeoengineeringWatch.org Extremely powerful ground based microwave transmission facilities are being used in conjunction with the ongoing atmospheric aerosol spraying operations to completely manipulate Earth’s climate -
Manufacturing Winter Weather, Engineering A “Bombogenesis” Cyclone
Dane Wigington GeoengineeringWatch.org Global climate engineering operations are not a proposal, they have been an ongoing reality for over 70 years. From weather warfare to engineered -
Climate Extremes, Geoengineering And Winter Weather Whiplash
Dane Wigington GeoengineeringWatch.org Erratic and unprecedented weather is radically increasing all over the world. Though there are countless forms of human activity affecting the equation, the global
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Engineering Wildfires
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Wildfires As A Weapon: US Military Exposed
Dane Wigington GeoengineeringWatch.org Is the military industrial complex insane enough to incinerate Earth's last remaining forests in order to achieve the objectives of the global controllers? The -
Unforeseen Circumstances, The Fawn Fire, Northern California
Dane Wigington GeoengineeringWatch.org Wildfire roulette continues to take a terrible toll in Northern California. The climate engineering cabal has relentlessly cut of the flow of moisture into -
Geoengineering: Fire And Ice
Dane Wigington GeoengineeringWatch.org Geoengineering is wreaking havoc all over the world, from fires to ice. After the passing of the recent engineered winter weather onslaught on Northern California, -
Engineering Catastrophic Wildfires To Temporarily Cool Arctic With Smoke (Updated)
Dane Wigington GeoengineeringWatch.org Are covert climate engineering operations connected to the exponentially increasing wildfires all over the world? June, 2019, was the hottest month ever recorded on our -
Geoengineering Creating Freeze Fry Extremes
Dane Wigington GeoengineeringWatch.org Global climate engineering operations are creating ever more extreme weather and temperature whiplash scenarios. The immense scale of climate forcing being carried out by -
Governor Newsom, Geoengineering And Unprecedented Wildfires
Dane Wigington GeoengineeringWatch.org Newly elected California governor Gavin Newsom has had to declare state of emergency due to the latest unprecedented firestorms. Will Newsom disclose the climate engineering factor that is -
Geoengineering Is Fueling Firestorm Catastrophes
Dane Wigington GeoengineeringWatch.org Yet again forests in the Western US are incinerating as the US east coast is anomalously wet and cool. All official sources are blaming -
Climate Engineering, Clearcutting, And Record Wildfires
Dane Wigington GeoengineeringWatch.org Record wildfires continue to sweep the globe, incinerating what yet remains of Earth’s formerly vast thriving forests. Why? We are told by official sources that -
California Firestorms: Geoengineered Catastrophe
Dane Wigington GeoengineeringWatch.org Yet another round of extreme firestorm catastrophes are hitting California. Like the recent Santa Rosa fire disaster, the current wildfires are verifiably connected to -
Climate Engineering Fuels Fires Of Global Incineration
Dane Wigington geoengineeringwatch.org Our once thriving planet is spiraling toward complete meltdown and total extinction. Though there are countless forms of anthropogenic damage to the biosphere (with
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Into The Wild
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Against All Odds, Survivors In A Dying Forest
Dane Wigington GeoengineeringWatch.org The climate engineers are contaminating and cutting off the life giving flow of rain to forests all over the world. Trees are dying and -
Domino Effect: Weather Warfare, Wasted Forests and Worldwide Collapse Of Ecosystems
Dane Wigington GeoengineeringWatch.org The global controllers are utilizing every tool they have at their disposal to distract populations from the wider horizon of unfolding and accelerating global -
Unforeseen Circumstances, The Fawn Fire, Northern California
Dane Wigington GeoengineeringWatch.org Wildfire roulette continues to take a terrible toll in Northern California. The climate engineering cabal has relentlessly cut of the flow of moisture into -
California Drought, How To Party On The Titanic
Dane Wigington GeoengineeringWatch.org Climate engineering operations are cutting off the flow of moisture to the US West, how much longer can Californians last without rain? Primary reservoirs -
Aftermath Of Chemical Ice Nucleation
Dane Wigington GeoengineeringWatch.org Chemically nucleated “snow” events are wreaking havoc on forests, ecosystems and human infrastructure. Chemical ice nucleation cloud seeding processes are a primary component of -
Geoengineering: Fire And Ice
Dane Wigington GeoengineeringWatch.org Geoengineering is wreaking havoc all over the world, from fires to ice. After the passing of the recent engineered winter weather onslaught on Northern California, -
Chemically Nucleated Winter Weather
Dane Wigington GeoengineeringWatch.org The extremely anomalous surface cool-down in Texas and other parts of the world are not just random acts of Nature. Global climate engineering operations are not just a -
Into The Wild, With Dane Wigington, September 16, 2020, #2
Dane Wigington GeoengineeringWatch.org How dire are conditions in our last remaining wilderness areas? What primary factors are fueling the record wildfires that are incinerating formerly thriving forests? What -
Into The Wild, With Dane Wigington, September 10, 2020, #1
Dane Wigington GeoengineeringWatch.org How dire are conditions in our last remaining wilderness areas? What primary factors are fueling the record wildfires that are incinerating formerly thriving forests? What
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OZONE DESTRUCTION
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Is It Game Over? New NASA Report
Dane Wigington GeoengineeringWatch.org Global climate engineering operations have been pushed and propagated on the premise of cooling the planet by saturating Earth's skies with sun blocking aerosols. -
Imminent Ozone Layer Collapse, A Dire Warning From A Former NASA Contract Engineer
Dane Wigington GeoengineeringWatch.org Does the sun feel scorching hot? A former aerospace avionics engineer who worked with NASA has been conducting testing for Geoengineering Watch with state -
UVC: The Sterilization Of Planet Earth, Part One
Dane Wigington GeoengineeringWatch.org Earth’s life preserving ozone layer is deteriorating at blinding speed, covert climate engineering operations are the single greatest causal factor. If this destruction is -
The Ozone Layer Recovery Lie And Climate Engineering Denial, Both Are Breaking Down
Dane Wigington GeoengineeringWatch.org Until recently official sources have pushed the patently false narrative that Earth's ozone layer was recovering, that blatant lie is now becoming impossible to maintain. Our planet's -
Ozone Layer Collapse, A Former NASA Contract Engineer Warns Of Planetary Omnicide
Dane Wigington GeoengineeringWatch.org We are told by “official” sources that the ozone layer is recovering, that we have nothing to worry about. This narrative is being pushed by -
Climate Engineering, Ozone Destruction, And Radiation Clouds, The Dangers Of Air Travel
Dane Wigington geoengineeringwatch.org Air travel is becoming ever more hazardous to human health for a host of reasons. Not only are air travelers inhaling high concentrations of toxic -
Geoengineering Is Accelerating A Runaway Greenhouse Scenario
Science circles and governments all over the globe are pushing the narrative that "geoengineering could mitigate global warming". This narrative is so astoundingly false from so many directions, the fact -
Climate Engineers Cut Off Critical Rain From Populations Around The Globe
Dane Wigington geoengineeringwatch.org Why are there so many record droughts unfolding around the world? A warmer atmosphere holds much more water vapor (7% more for every degree in
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HAARP
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Hurricane Helene And Frequency Transmissions, 90 Second Alert
Dane Wigington GeoengineeringWatch.org Was Hurricane Helene’s path and behavior just an act of nature? What do you think? Are networks of ground based frequency transmitters being utilized -
Hurricane Hilary: Failure Or Mission Accomplished?
Dane Wigington GeoengineeringWatch.org Hurricane Hilary was highly sensationalized by mainstream media, what agendas might Hurricane Hilary have served for those in power? Could Hilary have been utilized to -
Are Microwave Transmission Facilities Being Used To Trigger Earthquakes?
Dane Wigington GeoengineeringWatch.org To what degree can the seismic forces of nature be manipulated? Please watch this 4 minute video that will provide insight and points to ponder. All -
Geoengineering Microwave Transmissions And Their Connection To Hurricane Florence
Dane Wigington GeoengineeringWatch.org Every aspect of our weather is being altered and manipulated by geoengineering programs, including hurricanes / cyclones. Radio frequency / microwave transmission installations have -
Geoengineered Western US Meltdown To Cool The Rest Of The Country, Again
Dane Wigington GeoengineeringWatch.org The Western US has, at minimum, long since become a climate sacrifice zone for the geoengineers. The current NOAA departure from normal high temperature "forecast"
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Disinformation
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Mainstream Media Coverage Of Climate Engineering, What Is The Agenda?
Dane Wigington GeoengineeringWatch.org Imagine the whole of academia pretending the ongoing geoengineering operations we see in our skies are not really there. Imagine the vast majority of populations blindly -
Mainstream Media, CBS Australia, Covers Climate Engineering
Dane Wigington GeoengineeringWatch.org Corporate media, of course, does their best to spin and marginalize the geoengineering reality as they are paid and told to do. This being said, the -
Climate Engineering Disinformation, How To Respond To The Source
Dane Wigington GeoengineeringWatch.org The global climate engineering assault is becoming all but impossible to hide in plain sight as the climate and biosphere collapse accelerates. In response, the
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Global Meltdown
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Is It Game Over? New NASA Report
Dane Wigington GeoengineeringWatch.org Global climate engineering operations have been pushed and propagated on the premise of cooling the planet by saturating Earth's skies with sun blocking aerosols. -
Engineering Catastrophic Wildfires To Temporarily Cool Arctic With Smoke (Updated)
Dane Wigington GeoengineeringWatch.org Are covert climate engineering operations connected to the exponentially increasing wildfires all over the world? June, 2019, was the hottest month ever recorded on our -
Peat, The World’s Largest Terrestrial Carbon Store, Ignites Into ‘Unprecedented’ Arctic Firestorms
Source: Daily KOS Throughout the High Arctic of Canada, Alaska, and Siberia unprecedented wildfires are raging. Thanks to stunning satellite images from NASA and the EU of -
Fleeing Climate Change – The Real Environmental Disaster – DW Documentary
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Ten Times More Wildfires In Russian Arctic Than A Decade Ago As Ice Melts And Gives Way To Flame
Source: The Telegraph Russia this year has suffered the most Arctic wildfires since satellite monitoring started as climate change creates the conditions necessary for blazes to -
Methane Deathtrap Threatens Democracy
Source: Counterpunch.org A methane deathtrap – continuing Arctic Ocean eruption of ever-increasing levels of methane brings forth speculation of a “Black Swan Event,” meaning society is -
Rising Ocean Waters From Global Warming Could Cost Trillions Of Dollars
Source: The Guardian We’ll need to mitigate and adapt to global warming to avoid massive costs from sea level rise. Ocean waters are rising because of -
An Iceberg The Size Of Lower Manhattan Just Broke Off Greenland
Source: Earther.com Iceberg calving events are among the more epic spectacles on the planet. But rarely have humans been lucky enough to see them happen in -
A Grand Solar Minimum Would Barely Make A Dent In Human-Caused Global Warming
Source Skeptical Science What The Science Says: Peer-reviewed research, physics, and math all tell us that a grand solar minimum would have no more than a -
Biological Annihilation And The Sixth Mass Extinction
Source: Resilience Human beings are now waging war against life itself as we continue to destroy not just individual lives, local populations and entire species in
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The 36,000 member Institute of Physics
“Climate geoengineering at scale must be considered only as a last resort…There should be no lessening of attempts to otherwise correct the harmful impacts of human economies on the Earth’s ecology and climate.”
IS RAYTHEON THE WEATHER?
Raytheon Corporation is the third largest weapons manufacturer, and is a partner in HAARP. Raethon also tells the weather to the American Meteorological Service (AMS) and is the leading corporation in Weather Modification Nano Technology, as well as advanced Weather Weapon Systems. Here is Raytheon's RAY GUN.....crowd control, weather modification, weapons systems, weather forecasting.......Raytheon. Still think they can't alter the weather?
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Runaway Methane Scenario
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Engineering Catastrophic Wildfires To Temporarily Cool Arctic With Smoke (Updated)
Dane Wigington GeoengineeringWatch.org Are covert climate engineering operations connected to the exponentially increasing wildfires all over the world? June, 2019, was the hottest month ever recorded on our -
Methane Deathtrap Threatens Democracy
Source: Counterpunch.org A methane deathtrap – continuing Arctic Ocean eruption of ever-increasing levels of methane brings forth speculation of a “Black Swan Event,” meaning society is -
Global Weather Modification Assault Causing Climate Chaos And Environmental Catastrophe
Dane Wigington geoengineeringwatch.org Global climate engineering encompasses many different aspects and processes. The consequences of the climate engineering insanity are already unquantifiable and growing by the day. -
Geoengineering, Methane Eruptions, And Imploding Arctic Ice
Dane Wigington GeoengineeringWatch.org The East Siberian Sea is "boiling with methane", scientists have called what they are witnessing "truly terrifying". Massive methane eruptions are pushing the planet toward -
Is Climate Engineering Contributing To Noctilucent Cloud Formation?
Dane Wigington geoengineeringwatch.org The rapid expansion of noctilucent cloud formations and sightings is an ominous sign of our rapidly disintegrating biosphere that almost no one is heeding. -
Methane Plume Seen On Radar Returns Moving NW From Offshore SW Of San Francisco Moving Toward Reno, Nevada
Source: otterwalks Methane deposits are abundant on the continental margin of the Pacific Northwest coast as more methane is seen being released southward. It is calculated that -
Dead Fish, Dying Planet, And Dangerous Denial
Dane Wigington geoengineeringwatch.org Fish are dying, wildlife is dying, the entire web of life is dying, but willful denial of verifiable realities continues to thrive. The global -
Climate Engineering And The “Coming Ice Age” Narrative
Dane Wigington geoengineeringwatch.org When a society has been very successfully polarized by decades of carefully crafted propaganda, beliefs and wishful thinking can (and all too often do) -
Catastrophic Pacific Ocean Die-Off, The US Military’s All Out Assault On The Web Of Life
Dane Wigington geoengineeringwatch.org Many have heard about some of the die-offs occurring in our oceans, but most have no understanding of how catastrophic the actual reality is. -
Geoengineered Incineration Is Pushing The Planet Toward Venus Syndrome
Dane Wigington geoengineeringwatch.org Yet more forest land is being incinerated by raging, unprecedented, and completely out of control wildfires in the US West. Flames from the "Butte
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The Coming Collapse
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Engineering Catastrophic Wildfires To Temporarily Cool Arctic With Smoke (Updated)
Dane Wigington GeoengineeringWatch.org Are covert climate engineering operations connected to the exponentially increasing wildfires all over the world? June, 2019, was the hottest month ever recorded on our -
Ozone Layer Collapse, A Former NASA Contract Engineer Warns Of Planetary Omnicide
Dane Wigington GeoengineeringWatch.org We are told by “official” sources that the ozone layer is recovering, that we have nothing to worry about. This narrative is being pushed by -
False Flag Chemical Weapons Attack Pushes WWlll, While Global Geoengineering Omnicide Continues
Dane Wigington GeoengineeringWatch.org The US government and the western power structure as a whole is completely out of control. Though there is no denying the corruption and criminality -
USA Watchdog News Site Covers Critical Climate Engineering Issue
Greg Hunter is a highly respected veteran news reporter who has worked for many major news networks in the past. Greg continues to make his voice heard -
Environmental Collapse And The Unravelling Of Civilization
Even now the vast majority of first world nation populations are completely oblivious to the unfolding environmental cataclysms that are closing in from every side. This unimaginable
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Government Document “Geoengineering Governance and Technology”
OWNING THE WEATHER
View the Government Documents
In B.C Canada in 2021 we had what they called an atmospheric river. I always thought something was not rite. I watched the dimming a few months ago and learned even more than I thought they where doing. Dane was talking about the so called 5 g microwave towers. I was wondering if they where using graphine with geo engineering. Now I'm not an expert , but in my thought wave that would make the rain a conductor and there for weponizing those towers to do bad things with the rain like …….. just sayin.
Most-likely also be used to inventory the complete planet.
Everything, Micromanaged.
Ex. iot (IOT) manage planet resources.
Ex.
https://behrtech.com/blog/iot-and-sustainability-7-applications-for-a-greener-planet/
…
A "graphene sky" is reference to prophecies of Habakkuk. So the wealthy, lunatic controllers can sweep over the nations like locusts, stealing up all their "wealth". I.e., privacy and lives.
They are so relentlessly spraying over Germany these days ,there are over 20chemtrail lines over my house in North-Rhine Westfalen. Pure Horror.
Central Virginia gets heavily inundated with jet Chemtrails almost every single day. They must have made a modification to the technology because these new "clouds" are looking rather rippled and waveform-like, if that makes any sense.
I've only known about chemtrails since about 2008 and I've tried to tell people about it while most look at me like I have two heads.
If we only have until 2025, then I want to spend as much time as I can in church and with those I love the most!'
Absolutely correct.You have public science papers about advantages of graphene oxide in jet fuels.Want to report you that in eastern Europe they are spraying visible mash on our skies in last few years.Its getting worse.Incredible summer storms.Trees dying. Last days we have oddly clear blue sky.Why? Satanist are busy with war prepares over Ukraine.Resistance!
Numerous chemtrails have been injected into the Forest Lake, Minnesota skies nearly daily for the past year. I've also observed chemtrails on a regular basis over Coon Rapids, MN. I've taken hundreds of photos to document these trails and the odd cloud formations that follow them.
I noticed the alarming plaid layering of trails in the skys about a week before Christmas….a week later "Omicron" is rampant in our area… Hmmm interesting….
Dane, why don't you get in touch with the likes of David Attenborough and Greta thunberg – please. People need a leader, someone who has reputation, someone who is already widely known. That is our only option to spread te message across the world.
Hello, Hope, thank you for standing with us in this all important battle. Yes, I agree that such well known individuals need to get a heads up so that they start to look up. I have reached out to as many as possible and had Geoengineering Watch materials delivered to many, but I confess that I need all the help I can get with this task. I will continue to tend as many fires as I can in the battle, but there is just not enough time in a day for me. If we have any chance of reaching a critical mass of awareness in time, it will take our collective efforts, all of us.
https://www.geoengineeringwatch.org/how-do-we-stop-climate-engineering-only-way-forward/
David A. will not speak on this matter and Greta is entirely a puppet of the false Global Warming scare-mongering campaign. Only ordinary people can stop this dangerous geoengineering when enough numbers wake up to the reality.
The massive rainfall and floods in Sydney Australia this week was also preceded by spraying activity clearly visible for over a week. Now over 50,000 houses evacuated…
Hope, Attenborough and Thunberg are BOTH members of the WEF and NWO. They spout about climate change all the time….when the truth is this….our skies have been the target of spraying since 1932 when Leningrad started experimenting…
my sister lives in a mexican village border with arizona, yuma, and she is scared now because her body is completely magnetized like those pics we see of the vaccinated people though she is far from getting the jab! coins, cell phone, everything metal glues to her skin!!!
Its time we studied what makes human beings want to harm others. Our approach seems to be still compulsion – behaviour modification – when we know that each child needs to develop its unique self and learn to respect the selves of others. Compulsion only makes people angry – or deviously cooperative from fear of retaliation – like school children. Learning freedom with responsibility is the only way, but it has to have a foundation of meeting infancy needs for a mother – a known affectionate person who is always there. Innocence deprived of its entitlement becomes a diabolical spirit.
VERY precise and ABSOLUTELY COHERENT comment that goes to the ROOT of a culture where people are willing to harm others in having been raised parked in front of televisions and screens in absolute deprivation of the relational requirements of a developing neurology.
We are studying that. These people are sociopaths, psychopaths and narcissists. There is no cure; just stop glorifying them and choosing them to run things.
raymond
you want to move – necessities
spring water, lots of wild plants not south facing – too hot these days
susan ferguson thank you for so much info
Geoengineering as conveyed in this documentary, is without doubt a blatant and appalling Act of War against The People, The Planet and All Life as we know it!
Omnicide.
US used Graphene net which is an exceptional conductor and is a strategic weapon requiring Presidential Sign Off against Baghdad in 2002 as the entire city went dark and the fragments shorted out all attempts to bring electricity back to the city for years as only spots could be rebuilt since all of the infrastructure was not possible to clear or clean. It is 1 atom wide and created by DARPA and MIT in large sheets. The term to tear this netting is called fission.
To bad that this is not known except for the few of us that monitored this event. This will end up in the trash like all the rest. The US is not what you think it is or was. Owned by the Crown, the Temple Knights of St. John in the City of London Corporation of which the Inner temple and middle temple still exist.
Frederick Gates was the lead researcher from the Carnegie School, now Rockefeller University that created the extremely deadly Pneumonia, Meningitis "Vaccine" in 1918. I have the paper.
1818-1824 it was Cholera that these Saturn worshippers let loose in India and west Asia for UK and US for the Opium Wars to come.
VSF: This is only one company of many in the graphene business. There are five others that are recommended as stock-buys for investors. Applied Graphene Materials (AGM), Haydale Graphene Industries (HAYD), AIXTRON (AIXA), Applied Graphene Materials (AGM), ZEN Graphene Solutions (ZEN).
The Future of Graphene and 5G
Grolltex Manager, April 2nd, 2018
At the February Mobile World Congress, held in Barcelona, Spain, the close connection between graphene and 5G technology was one of the main sources of attraction. Both have been featured prominently in the news recently as new-age wonders, so it’s only natural that the two would at some point combine forces to produce some truly remarkable capabilities. That time appears to be in the very near future, as considerable research has been underway on ways that the two could support each other for mutual benefit.
Monolayer graphene has only been commercially available for about the last decade, but it has already made a powerful impact in the areas of high-speed photosensitivity, farming applications, flexible photodetectors, medical diagnostics, and water purification processes. 5G of course, has been hailed as the future of communications for several years, and is considered to be the enabling technology for both VR and AR. Some of the ongoing research has been related to finding ways that super-conductive, flexible monolayer graphene could be used to support the needs of 5G technology, to help finally achieve the breakthrough it needs.
Graphene and 5G Technology Combined
5G technology cannot simply be scaled up from previous technology to meet the demands of high-speed communications of the future – it needs an enabling technology. Enter monolayer graphene. By late 2017, a research team at Chalmers University in Sweden had developed a method of combining graphene flexibility with terahertz detection so as to make it possible to connect the Internet of Things (IoT), via high-bandwidth technologies available in 5G.
Another group of researchers comprised of Italian University specialists and commercial companies, have produced a flexible microwave device for communications which has applications for Wi-Fi, and which can also be used in millimeter wave (mmWave) technologies. And why is this important? It’s universally acknowledged that the true future of 5G technology lies in this millimeter wave technology.
Currently some of the 5G high-frequency components are made using gallium arsenide technology, but this is very costly, and has been one of the factors slowing the development of 5G. Graphene can be manufactured much more inexpensively, and its quality is at least comparable and often superior to gallium arsenide. The only thing which hinders the shift from gallium arsenide to graphene is the immaturity of the graphene supply chain – this is a technology barely 10 years old, and there are not a lot of manufacturers able to supply components in the quantity that would be needed.
The Future of Graphene
The versatility of graphene will become more and more evident in the next several years, as graphene will be used in the manufacture of a wide range of superior products such as water desalination membranes, medical diagnostic devices, smartphone displays, stretchable electronics, advanced solar cells, and others. Graphene is clearly the wave of the future, and it will be fascinating to observe its ongoing development.
Grolltex is the most advanced graphene producer in the U.S. Through patented transfer and processing methods, we are able to produce single layer graphene at a cost structure not previously known. There are currently more than 15,000 products under patent-pending status which are based on graphene technology, and that number will most assuredly be growing in the coming few years. To acquire high-quality graphene sheets for sale, or monolayer graphene, contact us with any questions you might have, or to place an order.
https://grolltex.com/the-future-of-graphene-and-5g/
• 08 May 2020
Graphene oxide-incorporated hydrogels for biomedical applications
• Jongdarm Yi,
• Goeun Choe,
• Junggeon Park &
• Jae Young Lee
Polymer Journal volume 52, pages 823–837 (2020)
Abstract
Graphene and graphene derivatives (e.g., graphene oxide (GO)) have been incorporated into hydrogels to improve the properties (e.g., mechanical strength) of conventional hydrogels and/or develop new functions (e.g., electrical conductivity and drug loading/delivery). Unique molecular interactions between graphene derivatives and various small or macromolecules enable the fabrication of various functional hydrogels appropriate for different biomedical applications. In this mini-review, we highlight the recent progress in GO-incorporated hydrogels for biomedical applications while focusing on their specific uses as mechanically strong materials, electrically conductive scaffolds/electrodes, and high-performance drug delivery vehicles.
https://www.nature.com/articles/s41428-020-0350-9
Properties of Graphene Oxide
One of the advantages of the gaphene oxide is its easy dispersability in water and other organic solvents, as well as in different matrixes, due to the presence of the oxygen functionalities. This remains as a very important property when mixing the material with ceramic or polymer matrixes when trying to improve their electrical and mechanical properties.
On the other hand, in terms of electrical conductivity, graphene oxide is often described as an electrical insulator, due to the disruption of its sp2 bonding networks. In order to recover the honeycomb hexagonal lattice, and with it the electrical conductivity, the reduction of the graphene oxide has to be achieved. It has to be taken into account that once most of the oxygen groups are removed, the reduced graphene oxide obtained is more difficult to disperse due to its tendency to create aggregates.
Functionalization of graphene oxide can fundamentally change graphene oxide’s properties. The resulting chemically modified graphenes could then potentially become much more adaptable for a lot of applications. There are many ways in which graphene oxide can be functionalized, depending on the desired application. For optoelectronics, biodevices or as a drug-delivery material, for example, it is possible to substitute amines for the organic covalent functionalization of graphene to increase the dispersability of chemically modified graphenes in organic solvents. It has also been proved that porphyrin-functionalized primary amines and fullerene-functionalized secondary amines could be attached to graphene oxide platelets, ultimately increasing nonlinear optical performance.
In order for graphene oxide to be usable as an intermediary in the creation of monolayer or few-layer graphene sheets, it is important to develop an oxidization and reduction process that is able to separate individual carbon layers and then isolate them without modifying their structure. So far, while the chemical reduction of graphene oxide is currently seen as the most suitable method of mass production of graphene, it has been difficult for scientists to complete the task of producing graphene sheets of the same quality as mechanical exfoliation, for example, but on a much larger scale. Once this issue is overcome, we can expect to see graphene become much more widely used in commercial and industrial applications.
https://www.graphenea.com/pages/graphene-oxide#.YN9U3x1lCGg
Nanomaterials-Based Electrochemical Immunosensors
by Zhenguo Zhang, Yulin Cong, Yichun Huang and Xin Du *
College of Life Sciences, Key Laboratory of Food Nutrition and Safety, Shandong Normal University, Jinan 250014, China
Abstract
With the development of nanomaterials and sensor technology, nanomaterials-based electrochemical immunosensors have been widely employed in various fields. Nanomaterials for electrode modification are emerging one after another in order to improve the performance of electrochemical immunosensors. When compared with traditional detection methods, electrochemical immunosensors have the advantages of simplicity, real-time analysis, high sensitivity, miniaturization, rapid detection time, and low cost. Here, we summarize recent developments in electrochemical immunosensors based on nanomaterials, including carbon nanomaterials, metal nanomaterials, and quantum dots. Additionally, we discuss research challenges and future prospects for this field of study. View
https://www.mdpi.com/2072-666X/10/6/397
Fabrication and Characterization of a Biomaterial Based on Extracellular-Vesicle Functionalized Graphene Oxide
Julia Driscoll, Anuradha Moirangthem, Irene K. Yan and Tushar Patel*
• Department of Transplantation, Mayo Clinic, Jacksonville, FL, United States
Mesenchymal stem cell (MSC) derived extracellular vesicles (EV) are emerging as acellular therapeutics for solid organ injury and as carriers for drug delivery. Graphene-based materials are novel two-dimensional crystal structure-based materials with unique characteristics of stiffness, strength and elasticity that are being explored for various structural and biological applications. We fabricated a biomaterial that would capture desirable properties of both graphene and stem cell derived EV.
Developmental Toxicity of GO-EV Treatment
To determine if GO and sGO-based biomaterials have in vivo effects, we evaluated their toxicity using zebrafish. Dechorionation and hatching in zebrafish takes place between 48 and 72 hpf. First, we monitored the hatching rate of the zebrafish starting at 45 hpf. In comparison to vehicle treated control zebrafish, there was a modest delay in the hatching rates of the zebrafish treated with 100 μg/mLGO and GO-EV, conversely, the rates in zebrafish treated with 10 μg/mL EV were accelerated (Figure 7). A similar acceleration in hatching was observed in the zebrafish treated with 10 μg/mL sGO, whereas the zebrafish treated with 10 μg/mL sGO-EV, 100 μg/mL sGO and 100 μg/mL sGO-EV all exhibited delayed hatching rates (Supplementary Figure 1). Next, we evaluated for the development of any malformations. In zebrafish exposed to GO-EV, yolk sac edema and pericardial edema were observed. Pericardial edema was also observed in the zebrafish treated with either low or high concentrations of sGO-EV. On the contrary, there were no malformations observed in the zebrafish treated with PBS, GO, or sGO at either 10 or 100 μg/mL. The heart rate varied considerably at different time points, but we did not observe any trends toward decreased heart rate in any treatment groups (data not shown). We further assessed survival of zebrafish at 168 hpf. A slight increase in mortality was observed in zebrafish treated with GO-EV, and with higher concentrations of sGO or sGO-EV but there was no lethality observed in zebrafish treated with PBS, GO or lower concentrations of sGO.
Discussion
In the present study we have developed a graphene oxide-based biomaterial synthesized by copper-catalyzed cycloaddition of azide tagged bone marrow derived-MSC-EV to alkyne functionalized graphene oxide. This novel biomaterial offers the ability to combine the structural physicochemical benefits of graphene with the biological effects of MSC-EV. MSC-EV retain the intrinsic therapeutic properties of their parent cells and have shown to be effective in promoting tissue repair and regeneration, mitigating oxidative stress and modulating immune cell activities.
The utility of MSC-EV as acellular therapeutics is being increasingly recognized. Their use is enhanced by several properties. Their cargo can be altered by exogenous loading to selectively enrich them with modulatory agents such as anti-sense oligonucleotides (George et al., 2018), miRNAs (Pomatto et al., 2019) or siRNAs (Matsuda et al., 2019). In addition, selective manipulation of their content is feasible through genetic engineering of donor cells to express RNA or proteins of interest. Moreover, their surface can be engineered to express specific markers that facilitate tissue- or cell-targeted delivery of the EV. Furthermore, their cellular production can be modulated by microenvironmental perturbations (Yan et al., 2017; Zhang et al., 2020). Of particular therapeutic relevance, MSC-EV also retain an ability to home to sites of inflammation and injury, similar to their parental cells (Lai et al., 2013). For all of these reasons, MSC-EV are attractive acellular therapeutics as well as therapeutic delivery vehicles with the capacity for targeted delivery of bioactive therapeutic molecules.
[Theranostics is a term derived from a combination of the words therapeutics and diagnostics. In this emerging field of medicine, drugs and/or techniques are uniquely combined to simultaneously or sequentially diagnose and treat medical conditions.
The ability to acquire a diagnosis and administer therapy in one package is a game-changer for medicine as we know it. Not only does this offer the opportunity to save time and money, but it also potentially allows one to bypass some of the undesirable biological effects that may arise when these strategies are employed separately.
Theranostics and Nanomedicine
The medical application of nanotechnology is referred to as nanomedicine, and this is made possible by a vast range of medical and scientific methodologies. One such approach is the use of nanoparticles in theranostics. The ultimate aim of combining nanomedicine and theranostics is to modify disease and patient-specific outcomes in such a way that they are drastically improved. https://www.news-medical.net/health/What-is-Theranostics.aspx ]
The unique physicochemical properties and adaptability of graphene makes it attractive for development as a theranostic nanomaterial. Several biomedical applications such as drug and nucleic acid delivery, biosensing, photothermal, photodynamic therapy and tissue engineering have been proposed for graphene and its derivatives such as GO and rGO (Robinson et al., 2011; Hu et al., 2012; Lee et al., 2016; Shin et al., 2016; Vinothini et al., 2019). The oxygen-containing functional groups in GO and rGO contribute to their overall colloidal stability in aqueous solutions. These derivatives are often conjugated to polymers or other biomolecules in order to mitigate membrane-damaging effects or the effects of oxidative stress. Similarly, the conjugation of MSC-EV to GO may permit additional properties that can be exploited toward broader potential biomedical applications. The MSC-EV cargo contains a variety of bioactive molecules that can work alone, or in concert, to elicit a therapeutic effect (Liang et al., 2016; Yan et al., 2017). The modifiability of the EV cargo and the EV surface profile can contribute to achieve the desired biological effects in a targeted fashion (Ye et al., 2018). Furthermore, considering that EV and GO are internalized by different mechanisms, conjugation of EV to GO could enhance GO uptake by recipient cells (Huang et al., 2012; McKelvey et al., 2015). Thus, the biological effects of MSC-EV such as reducing tissue injury can be coupled with physical, biochemical or structural functionalities offered by graphene.
An advantage of conjugation of EV to GO allows exploitation of the properties of graphene, such as surface modifications for additional functional properties. For example, cytotoxic effects of GO-EV could be augmented by loading chemotherapeutic that are released in a pH-responsive manner in tumor settings (Ardeshirzadeh et al., 2015; Wang et al., 2019). Other potential applications may involve fashioning the GO-EV as a structural biomolecule for implantation as an extracellular scaffold within tissues such as bone or teeth, or within endoprostheses and stents placed in the body (Diomede et al., 2018; Li et al., 2018). In this context, the ability to selectively load MSC-EV exogenously after isolation, or endogenously through genetic or protein manipulation of the parental cells offer the potential ability to use GO-EV as a therapeutic delivery platform. For such applications, further studies to determine the kinetics of EV release from GO-EV would be valuable to determine whether controlled release of MSC-EV can be accomplished for therapeutic benefit.
The paucity of developmental or genotoxic effects of the GO-EV biomaterial paves the way for development of further applications in tissue engineering and regenerative medicine. Within the context of bone tissue engineering, GO elicits pro-osteoneogenic effects in vitro and in vivo (Hermenean et al., 2017). BM MSC-EV cargo has been shown to be enriched in several pro-osteogenic miRNAs (Ardeshirzadeh et al., 2015). Thus, GO-EV could augment the osteoinductive effects observed with GO. We observed variable effects on cytotoxicity of GO-EV and sGO-EV in liver cancer cell lines, though minimal cytotoxicity was observed in healthy hepatocytes. Notably, no genotoxicity was observed. Moreover, we did not detect any significant developmental toxicity in zebrafish. sGO-EV and GO-EV are readily recognized and phagocytosed by macrophages. Following their internalization by RAW264.7 cells, an alteration in the secretome profile with enhanced secretion of the pro-inflammatory cytokine, TNF-α was observed. The immunological impact of these biomaterials warrants further evaluation. Biocompatibility in vivo could be improved via the conjugation of GO with polymers that are capable of being cleaved upon internalization of the biomaterial to prevent the adverse accumulation of GO in cells (Li et al., 2014). Additional surface modifications may further reduce undesirable immune effects observed in our in vitro study.
This study developed a process for fabrication of a graphene-based biomaterial incorporating MSC-EV and examined their cytotoxicity and immunologic effects in vitro and developmental toxicity effects in vivo. GO-EV induced an inflammatory response and cell-specific cytotoxicity. While some developmental malformations were observed, these had a minimal impact on overall survival in zebrafish. There are opportunities to further improve the biocompatibility of GO-EV. For example, variable effects of cytotoxicity have been observed with GO in different study settings. Cytotoxicity can be influenced by the flake size and the degree of oxygenation of GO, with the smaller and more oxygenated forms of GO eliciting more potent cytotoxic effects (Pelin et al., 2017; Gurunathan et al., 2019). Differences in GO induced cytotoxicity have been observed between different malignant and non-malignant cells (Fiorillo et al., 2015). Attention to optimized approaches and selection of base materials is warranted in future studies because cytotoxic effects could be impacted by the physical differences in the lateral dimensions and overall shapes of graphene noted between top-down and bottom-up synthetic approaches (Lee et al., 2019). Such efforts are warranted to take full advantage of the use of GO-EV as a functional biomaterial that combines the versatility of graphene with the intrinsic therapeutic effects of cell derived EV for the development of biomedical applications.
Data Availability Statement
The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author/s.
Ethics Statement
The animal study was reviewed and approved by the Mayo Clinic Institutional Animal Care and Use Committee.
https://www.frontiersin.org/articles/10.3389/fbioe.2021.686510/full
Graphene Flagship
Funded by the European Commission, the Graphene Flagship aims to secure a major role for Europe in the ongoing technological revolution, helping to bring graphene innovation out of the lab and into commercial applications.
https://graphene-flagship.eu/
Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms
• Lingling Ou,
• Bin Song,
• Huimin Liang,
• Jia Liu,
• Xiaoli Feng,
• Bin Deng,
• Ting Sun &
• Longquan Shao
… several typical mechanisms underlying GFN toxicity have been revealed, for instance, physical destruction, oxidative stress, DNA damage, inflammatory response, apoptosis, autophagy, and necrosis …
GFNs can be delivered into bodies by intratracheal instillation [30], oral administration [31], intravenous injection [32], intraperitoneal injection [33] and subcutaneous injection …
GFNs can induce acute and chronic injuries in tissues by penetrating through the blood-air barrier, blood-testis barrier, blood-brain barrier, and blood-placenta barrier etc. and accumulating in the lung, liver, and spleen etc. …
… graphene nanomaterials aerosols can be inhaled and substantial deposition in the respiratory tract, and they can easily penetrate through the tracheobronchial airways …
The toxicological mechanisms of GFNs demonstrated in recent studies mainly contain inflammatory response, DNA damage, apoptosis, autophagy and necrosis etc. …
GO can result in acute inflammation response and chronic injury …
Fibrosis and inflammation …
The GFNs exert different toxicological effects on male or female reproductive system. pregnant mice had abortions at all dose, and most pregnant mice died when the high dose …
The developmental toxicity of GFNs may induce structural abnormalities, growth retardation, behavioural and functional abnormalities, and even death. …
The cytotoxicity of GFNs in vitro has been verified in various cells to change the cell viability and morphology, destroy the membrane integrity, and induce DNA damage [110–112]. GO or rGO decrease cell adhesion; induce cell apoptosis; and enter lysosomes, mitochondria, cell nuclei, and endoplasm [113]. GQDs entered cells and induced DNA damage …
Graphene can increase cell viability [117] or cause cell death …
… cause dose-dependent toxicity … The high content of GO mainly deposited in the lungs, liver, spleen, and kidneys and was difficult to be cleaned by the kidneys …
Increasing concentrations of GO entered the lysosomes, mitochondria, endoplasm, and cell nucleus …
GO can insert between the base pairs of double-stranded DNA and disturb the flow of genetic information at the molecular level, which might be one of the main causes of the mutagenic effect of GO …
… the importance of the GO surface charge because of its ability to affect the internalization and uptake mechanism of cells …
… strong electrostatic interactions between the negatively charged oxygen groups on the GO/GS surface and positively charged phosphatidylcholine lipids on the RBC outer membrane …
The physical interaction of graphene nanoparticles with cell membranes is one of the major causes of graphene cytotoxicity …
… the sharpened edges of GNS may act as ‘blades’, inserting and cutting through bacterial cell membranes …
… overwhelm the activity of antioxidant enzymes, including catalase, SOD, or glutathione peroxidase (GSH-PX) … The interactions of GO with cells can lead to excessive ROS generation, which is the first step in the mechanisms of carcinogenesis, ageing, and mutagenesis …
Exposure to GFNs resulted in significantly increased coupled and uncoupled mitochondrial oxygen consumption, dissipation of the mitochondrial membrane potential, and eventual triggering of apoptosis by activating the mitochondrial pathway …
GFNs can cause apoptosis and/or cell necrosis by direct influencing cell mitochondrial activity …
Due to its small size, high surface area and surface charge, GO may possess significant genotoxic properties and cause severe DNA damage, for example, chromosomal fragmentation, DNA strand breakages, point mutations, and oxidative DNA adducts and alterations …
Even if GO cannot enter into the nucleus of a cell, it may still interact with DNA during mitosis when the nuclear membrane breaks down, which increases the opportunity for DNA aberrations …
The π stacking interaction between the graphene carbon rings and the hydrophobic DNA base pairs can make a DNA segment ‘stand up’ or ‘lay on’ the surface of graphene with its helical axis perpendicular or parallel, respectively. The intermolecular forces severely deform the end base pairs of DNA, which potentially increases the genotoxicity …
DNA damage can not only initiate cancer development but also possibly threaten the health of the next generation if the mutagenic potential of GO arises in reproductive cells, which impacts fertility and the health of offspring …
A strong inflammatory response was induced by subcutaneously injection …
Apoptosis is defined as the self-destruction of a cell regulated by genes through complicated programmes … after inhalation …
… graphene and GO physically damaged cell membranes … increased the permeabilization of the outer mitochondrial membrane and changed the mitochondrial membrane potential … triggered by the death-receptor and canonical mitochondrial pathway …
Necrosis is an alternate form of cell death induced by inflammatory responses or cellular injury. The exposure of cells to pristine graphene causes apoptosis and necrosis at high doses …
GFNs could cause subtle changes in gene expression programming by modulating epigenetic changes.
https://pubmed.ncbi.nlm.nih.gov/27502632/
This is madness, in the quest to create their Internet of Things, with controllable Human cyborgs being just another Thing, they are seriously contaminating the Earth…I wonder what Enki thinks of this ?
So, the internet of things, is worth all of our lives? Or are we supposed to become part of the Borg? Either way, they are screwing us all up. Why because of communication junkies? Or they are too lazy to wait for information to download?
I fail to understand why people think this ok. It's not ok! ok!
I'm guessing that they probably used Graphene for this engineered winter weather event, to likely destroy the world's largest coffee bean crops. IN BRAZIL?
Photos show snow on Brazil streets during a rare winter weather phenomenon (msn.com)
"Some Brazilians tweeted their reaction on the winter phenomenon, saying the snowfall brought 'intense cold air-break' and hail."
Sounds exactly like the intense cold air-break and hail phenomenon we had here in Texas back in February!
Ironic how most of these engineered winter weather and heat dome events, only seem to occur where crops, vineyard, orchards and other agricultural based economies are located. Or major gas, coal and oil refineries are operating. Plus the "mystery" cyber attacks on pipelines and meat processing plants.
Anyone else seeing a pattern developing here, besides just Dane?
Modern and historical warfare tactics include crippling the civilian populations and I find it hard to believe that Nature would be just as discerning. Because the military industrial complex continues to grow stronger with each passing weather (Climate) anomaly. While the general public starves, freezes or suffers from heat stroke!
In their book, all of this is considered "Mission Accomplished". Especially when it can all be done covertly, while operating right out in plain sight as well.
Thank you Dane. I mentioned to you on a You Tube post I'd been smelling aluminum in my sinuses on heavily cloudy days. Now I know why.
It's too bad more of my acquaintances can't seem to wrap their heads around what you've been informing me about for years.
I was wondering why the skies look different as of late. They spray very early now in the morning. It is stringy clouds and they burn off by midday. There is still a lot of aluminum because I see it sparkling in sunlight. Graphene, wow, our world covered in it and our bodies injected with it. You cannot make this evil up!
Thank You Dane!!! This absolutely confirms what I've been telling others about viruses amidst the other toxins in the spraying. It's even effected my little dog as she has developed a chronic cough that gets worse when we've spent time outdoors.
I was able to watch this on my Roku TV 'You Tube' App but not on my iPhone You Tube App. That App is playing every other video with similar titles and wording but not showing this video. APPLE is definitely not getting positive feedback on this, from me! I hope it stays on You Tube for everyone to watch!
Thank You again for this Vital Information Update!!!
RandyJ